High-throughput, Microscale Magnetic Bead Protein Purification on the Ginkgo Reconfigurable Automation Cart (RAC) Platform

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Introduction

Authors: Tim Curran, Ph.D., Anna Zmich, Ph.D., Abinaya Badri, Ph.D.

This technology note discusses the onboarding of a simple, tip-based, generalized magnetic bead protein purification workflow onto Ginkgo’s RAC platform. Configured through Ginkgo’s Catalyst software, our protocol supports diverse magnetic bead-based purifications with different bind, wash, and elute schemes.

Using only a minimal device set centered around a compact 96-well liquid handling system, the system processes 96 cell lysate samples to yield 10s–100s µg purified protein in ~1 hour and, unlike typical large-deck liquid handling system implementations, is straightforward to scale and requires no hands-on involvement during runtime.

Outcomes

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